Journal: Neuron

Article Title: Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity

doi: 10.1016/j.neuron.2022.09.021

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies used for immunoblotting were: goat anti-PlexinD1 (1:500-1:800, R&D systems Cat# AF4160), rabbit anti-GAPDH (1:10,000, Cell Signaling Cat# 2118), rabbit anti pan-Actin (1:1000; Cell Signaling Cat# 4968), rat anti-Semaphorin3C (1:500; R&D Systems Cat# MAB1728). . Quantitative RT-PCR RNA was extracted from the dorsal and ventral spinal cord of E12.5 embryos with RNeasy Mini Kit (Qiagen). cDNA was synthesized from 500ng RNA with M-MLV reverse transcriptase (Thermo Fisher Scientific Cat# 28025013) and random primers (Thermo Fisher Scientific Cat# N8080127), diluted to 40μl in molecular biology-grade water and used for real-time quantitative PCR with SYBR Select Master Mix (Thermo Fisher Scientific Cat#4472913).

Techniques: Recombinant, Protease Inhibitor, Transfection, Plasmid Preparation, Clone Assay, Mutagenesis, Reverse Transcription, Sample Prep, Knock-Out, Control, Quantitative RT-PCR, In Situ, Software, Expressing, CRISPR, RNAscope

Journal: Neuron

Article Title: Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity

doi: 10.1016/j.neuron.2022.09.021

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies used for immunoblotting were: goat anti-PlexinD1 (1:500-1:800, R&D systems Cat# AF4160), rabbit anti-GAPDH (1:10,000, Cell Signaling Cat# 2118), rabbit anti pan-Actin (1:1000; Cell Signaling Cat# 4968), rat anti-Semaphorin3C (1:500; R&D Systems Cat# MAB1728). . Quantitative RT-PCR RNA was extracted from the dorsal and ventral spinal cord of E12.5 embryos with RNeasy Mini Kit (Qiagen). cDNA was synthesized from 500ng RNA with M-MLV reverse transcriptase (Thermo Fisher Scientific Cat# 28025013) and random primers (Thermo Fisher Scientific Cat# N8080127), diluted to 40μl in molecular biology-grade water and used for real-time quantitative PCR with SYBR Select Master Mix (Thermo Fisher Scientific Cat#4472913).

Techniques: Recombinant, Protease Inhibitor, Transfection, Plasmid Preparation, Clone Assay, Mutagenesis, Reverse Transcription, Sample Prep, Knock-Out, Control, Quantitative RT-PCR, In Situ, Software, Expressing, CRISPR, RNAscope